Interactions between carbon nanotubes and DNA polymerase and restriction
endonucleases

doi:10.1088/0957-4484/18/2/025102

Interactions between carbon nanotubes and DNA polymerase and restriction endonucleases

Changqing Yi et al 2007 Nanotechnology 18 025102 (6pp) doi: 10.1088/0957-4484/18/2/025102

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Changqing Yi¹, Chi-Chun Fong¹, Weiwei Chen², Suijian Qi¹, Chi-Hung Tzang¹, Shuit-Tong Lee^2,3 and Mengsu Yang^1,3
¹ Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong SAR, People's Republic of China
² Center of Super-Diamond and Advanced Films and Department of Physics and Materials Science, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong SAR, People's Republic of China
³ Authors to whom any correspondence should be addressed
E-mail: apannale@cityu.edu.hk (S-T Lee) and bhmyang@cityu.edu.hk (M Yang)

Abstract. Effects of multi-walled carbon nanotubes (MWCNT) and single-walled carbon nanotubes (SWCNT) functionalized with and without carboxylic groups on polymerase chain reaction (PCR) and restriction digestion reaction were investigated. The results showed that CNT can reduce and even inhibit PCR and restriction digestion reaction, possibly due to the decrease of respective enzyme activity. The inhibition effect on double restriction digestion reaction and PCR was increased in the order of CNT–COOH > pristine CNT and SWCNT> MWCNT. This study demonstrated that CNT may significantly affect the efficiency of biochemical reactions through different action mechanisms, which is critical for understanding how nanomaterials impact biological systems.

Print publication: Issue 2 (17 January 2007)
Received 14 September 2006, in final form 6 November 2006
Published 15 December 2006

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Interactions between carbon nanotubes and DNA polymerase and restriction endonucleases

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