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RAPID COMMUNICATION

A new sub-nanosecond LED at 280 nm: application to protein fluorescence

C D McGuinness et al 2004 Meas. Sci. Technol. 15 L19-L22   doi: 10.1088/0957-0233/15/11/L02  Help

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C D McGuinness1, K Sagoo2, D McLoskey2 and D J S Birch1,2
1 Photophysics Group, Department of Physics, University of Strathclyde, Glasgow G4 0NG, UK
2 Horiba Jobin Yvon IBH Ltd, Skypark 5, 45 Finnieston Street, Glasgow G3 8JU, UK
E-mail: djs.birch@strath.ac.uk

Abstract. We demonstrate measurement of the intrinsic fluorescence decay of a protein excited with a new and inexpensive optical source based on a light emitting diode (LED) giving 600 ps pulses at ~280 nm. We believe this source will offer significant new capabilities for fluorescence research and development.

Keywords: LED, light emitting diode, protein fluorescence, pulsed optical sources, sensors, fluorescence decay time, fluorescence lifetime

Print publication: Issue 11 (November 2004)
Received 9 August 2004, in final form 14 September 2004
Published 11 October 2004

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